Isoniazida
Descripción general
Descripción
Isoniazida, también conocida como hidrazida del ácido isonicotínico, es un agente antimicrobiano sintético que se utiliza principalmente en el tratamiento y la prevención de la tuberculosis. Se sintetizó por primera vez en 1912 y se introdujo en la práctica clínica en la década de 1950. La this compound es un medicamento antituberculoso de primera línea debido a su alta eficacia y selectividad contra Mycobacterium tuberculosis, la bacteria responsable de la tuberculosis .
Mecanismo De Acción
La isoniazida es un profármaco que requiere activación por la enzima bacteriana catalasa-peroxidasa (KatG). Una vez activada, inhibe la síntesis de ácidos micólicos, componentes esenciales de la pared celular micobacteriana. Esta inhibición altera la síntesis de la pared celular, lo que lleva a la muerte de las células bacterianas. La this compound también interfiere con la síntesis de ADN, lípidos, carbohidratos y dinucleótido de adenina y nicotinamida (NAD), contribuyendo a sus efectos bactericidas .
Compuestos Similares:
Rifampicina: Otro fármaco antituberculoso de primera línea que inhibe la síntesis del ARN bacteriano.
Etambutol: Inhibe la síntesis de la pared celular micobacteriana al atacar las arabinosiltransferasas.
Pirazinamida: Altera el metabolismo y las funciones de transporte de la membrana celular micobacteriana
Singularidad de la this compound: La singularidad de la this compound radica en su mecanismo de acción específico, que se dirige a la síntesis de ácidos micólicos, que es crucial para la pared celular micobacteriana. Su capacidad para utilizarse tanto como monoterapia para la tuberculosis latente como en combinación con otros fármacos para la tuberculosis activa la convierte en un medicamento versátil y esencial en el tratamiento de la tuberculosis .
Aplicaciones Científicas De Investigación
La isoniazida tiene una amplia gama de aplicaciones de investigación científica:
Química: Se utiliza como reactivo en la síntesis orgánica y como precursor para la síntesis de diversos derivados.
Biología: Se estudia por sus efectos sobre la síntesis de la pared celular bacteriana y su papel en los mecanismos de resistencia bacteriana.
Medicina: Se utiliza principalmente en el tratamiento y la prevención de la tuberculosis. .
Industria: Se utiliza en la producción de productos farmacéuticos y como intermedio en la síntesis de otros compuestos químicos
Análisis Bioquímico
Biochemical Properties
Isoniazid plays a crucial role in biochemical reactions, particularly in the inhibition of mycolic acid synthesis, which is essential for the bacterial cell wall. Isoniazid is a prodrug that requires activation by the bacterial enzyme catalase-peroxidase (KatG). Upon activation, isoniazid forms an adduct with nicotinamide adenine dinucleotide (NAD), which subsequently inhibits the enzyme InhA, an enoyl-acyl carrier protein reductase involved in mycolic acid synthesis . This inhibition disrupts the synthesis of mycolic acids, leading to the death of the mycobacteria.
Cellular Effects
Isoniazid exerts significant effects on various types of cells and cellular processes. In Mycobacterium tuberculosis, isoniazid inhibits cell wall synthesis, leading to cell lysis and death. In human cells, isoniazid can cause hepatotoxicity, which is believed to be mediated by the formation of reactive metabolites through the cytochrome P450 enzyme system . These metabolites can induce oxidative stress and damage cellular components, including lipids, proteins, and DNA. Additionally, isoniazid has been associated with pyridoxine (vitamin B6) deficiency, as it increases the excretion of pyridoxine, affecting cellular metabolism .
Molecular Mechanism
The molecular mechanism of isoniazid involves its activation by the bacterial enzyme KatG. The activated form of isoniazid interacts with NAD to form an adduct that inhibits the enzyme InhA . This inhibition prevents the synthesis of mycolic acids, which are vital components of the mycobacterial cell wall. The disruption of mycolic acid synthesis leads to the loss of cell wall integrity and ultimately the death of the bacterium. Additionally, isoniazid can induce the expression of genes involved in oxidative stress response, further contributing to its bactericidal effects .
Temporal Effects in Laboratory Settings
In laboratory settings, the effects of isoniazid can change over time. Isoniazid is known to be relatively stable under standard storage conditions, but it can degrade when exposed to light and moisture . Over time, the degradation products of isoniazid can reduce its efficacy. Long-term exposure to isoniazid has been associated with hepatotoxicity in both in vitro and in vivo studies . The hepatotoxic effects are believed to be due to the accumulation of reactive metabolites that induce oxidative stress and liver damage.
Dosage Effects in Animal Models
The effects of isoniazid vary with different dosages in animal models. At therapeutic doses, isoniazid effectively inhibits the growth of Mycobacterium tuberculosis. At higher doses, isoniazid can cause toxic effects, including hepatotoxicity and neurotoxicity . In animal studies, high doses of isoniazid have been shown to induce liver damage, characterized by elevated liver enzymes and histopathological changes . Additionally, chronic administration of high doses of isoniazid can lead to peripheral neuropathy, which is attributed to pyridoxine deficiency .
Metabolic Pathways
Isoniazid undergoes extensive metabolism in the liver. The primary metabolic pathway involves acetylation by N-acetyltransferase 2 (NAT2) to form N-acetylisoniazid . N-acetylisoniazid is further hydrolyzed to isonicotinic acid and monoacetylhydrazine. Monoacetylhydrazine can be oxidized by cytochrome P450 enzymes to form reactive metabolites that contribute to hepatotoxicity . The rate of acetylation varies among individuals, leading to differences in drug clearance and susceptibility to adverse effects .
Transport and Distribution
Isoniazid is well-absorbed from the gastrointestinal tract and is widely distributed throughout the body, including the central nervous system . It has low protein binding and can penetrate tissues and cells effectively. Isoniazid is transported into cells via passive diffusion and is distributed to various tissues, including the liver, lungs, and kidneys . The distribution of isoniazid is influenced by its lipophilicity and the presence of transporters that facilitate its uptake into cells .
Subcellular Localization
Within cells, isoniazid is primarily localized in the cytoplasm, where it undergoes metabolic activation and exerts its effects . The activated form of isoniazid can interact with various cellular components, including enzymes and proteins involved in oxidative stress response . Additionally, isoniazid can induce the expression of genes involved in the detoxification of reactive metabolites, further influencing its subcellular localization and activity .
Métodos De Preparación
Rutas sintéticas y condiciones de reacción: La isoniazida se sintetiza típicamente mediante la reacción del ácido isonicotínico con hidrato de hidrazina. El proceso implica la esterificación del ácido isonicotínico con un alcohol y un agente de acilación para formar éster de ácido isonicotínico. Este éster se hace reaccionar entonces con hidrato de hidrazina para producir this compound .
Métodos de producción industrial: En entornos industriales, la preparación de la this compound implica los siguientes pasos:
Esterificación: El ácido isonicotínico se esterifica con un alcohol (por ejemplo, metanol) en presencia de un catalizador ácido.
Hidrazinolisis: El éster se hace reaccionar entonces con hidrato de hidrazina en condiciones de temperatura controlada para obtener this compound.
Purificación: El producto crudo se purifica mediante recristalización, decoloración y lavado para alcanzar una alta pureza y rendimiento
Análisis De Reacciones Químicas
Tipos de reacciones: La isoniazida se somete a diversas reacciones químicas, entre ellas:
Oxidación: La this compound puede oxidarse para formar ácido isonicotínico.
Reducción: Puede reducirse para formar derivados de hidrazina.
Sustitución: La this compound puede participar en reacciones de sustitución nucleófila
Reactivos y condiciones comunes:
Oxidación: El permanganato de potasio o el peróxido de hidrógeno pueden utilizarse como agentes oxidantes.
Reducción: El borohidruro de sodio o el hidruro de aluminio y litio pueden utilizarse como agentes reductores.
Sustitución: Los haluros de alquilo o los cloruros de acilo pueden utilizarse para reacciones de sustitución nucleófila
Productos principales:
Oxidación: Ácido isonicotínico.
Reducción: Derivados de hidrazina.
Sustitución: Diversos derivados sustituidos del ácido isonicotínico
Comparación Con Compuestos Similares
Rifampicin: Another first-line antituberculosis drug that inhibits bacterial RNA synthesis.
Ethambutol: Inhibits the synthesis of the mycobacterial cell wall by targeting arabinosyl transferases.
Pyrazinamide: Disrupts mycobacterial cell membrane metabolism and transport functions
Uniqueness of Isoniazid: Isoniazid’s uniqueness lies in its specific mechanism of action, targeting mycolic acid synthesis, which is crucial for the mycobacterial cell wall. Its ability to be used both as a monotherapy for latent tuberculosis and in combination with other drugs for active tuberculosis makes it a versatile and essential medication in tuberculosis treatment .
Propiedades
IUPAC Name |
pyridine-4-carbohydrazide |
Source
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|---|---|---|
| Source | PubChem | |
| URL | https://pubchem.ncbi.nlm.nih.gov | |
| Description | Data deposited in or computed by PubChem | |
InChI |
InChI=1S/C6H7N3O/c7-9-6(10)5-1-3-8-4-2-5/h1-4H,7H2,(H,9,10) |
Source
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| Source | PubChem | |
| URL | https://pubchem.ncbi.nlm.nih.gov | |
| Description | Data deposited in or computed by PubChem | |
InChI Key |
QRXWMOHMRWLFEY-UHFFFAOYSA-N |
Source
|
| Source | PubChem | |
| URL | https://pubchem.ncbi.nlm.nih.gov | |
| Description | Data deposited in or computed by PubChem | |
Canonical SMILES |
C1=CN=CC=C1C(=O)NN |
Source
|
| Source | PubChem | |
| URL | https://pubchem.ncbi.nlm.nih.gov | |
| Description | Data deposited in or computed by PubChem | |
Molecular Formula |
C6H7N3O |
Source
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| Record name | ISONIAZID | |
| Source | CAMEO Chemicals | |
| URL | https://cameochemicals.noaa.gov/chemical/20540 | |
| Description | CAMEO Chemicals is a chemical database designed for people who are involved in hazardous material incident response and planning. CAMEO Chemicals contains a library with thousands of datasheets containing response-related information and recommendations for hazardous materials that are commonly transported, used, or stored in the United States. CAMEO Chemicals was developed by the National Oceanic and Atmospheric Administration's Office of Response and Restoration in partnership with the Environmental Protection Agency's Office of Emergency Management. | |
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| Record name | ISONIAZID (OBSOLETE) | |
| Source | ILO-WHO International Chemical Safety Cards (ICSCs) | |
| URL | https://www.ilo.org/dyn/icsc/showcard.display?p_version=2&p_card_id=1258 | |
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| Explanation | Creative Commons CC BY 4.0 | |
| Record name | isoniazid | |
| Source | Wikipedia | |
| URL | https://en.wikipedia.org/wiki/Isoniazid | |
| Description | Chemical information link to Wikipedia. | |
| Source | PubChem | |
| URL | https://pubchem.ncbi.nlm.nih.gov | |
| Description | Data deposited in or computed by PubChem | |
DSSTOX Substance ID |
DTXSID8020755 |
Source
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| Record name | Isoniazid | |
| Source | EPA DSSTox | |
| URL | https://comptox.epa.gov/dashboard/DTXSID8020755 | |
| Description | DSSTox provides a high quality public chemistry resource for supporting improved predictive toxicology. | |
Molecular Weight |
137.14 g/mol |
Source
|
| Source | PubChem | |
| URL | https://pubchem.ncbi.nlm.nih.gov | |
| Description | Data deposited in or computed by PubChem | |
Physical Description |
Isoniazid appears as odorless colorless or white crystals or white crystalline powder. Taste is slightly sweet at first and then bitter. pH (1% aqueous solution) 5.5-6.5. pH (5% aqueous solution) 6-8. (NTP, 1992), Solid, WHITE CRYSTALLINE ODOURLESS POWDER. |
Source
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| Record name | ISONIAZID | |
| Source | CAMEO Chemicals | |
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| Record name | Isoniazid | |
| Source | Human Metabolome Database (HMDB) | |
| URL | http://www.hmdb.ca/metabolites/HMDB0015086 | |
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| Record name | ISONIAZID (OBSOLETE) | |
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Flash Point |
374 °F (NTP, 1992), > 250 °C |
Source
|
| Record name | ISONIAZID | |
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| Record name | ISONIAZID (OBSOLETE) | |
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Solubility |
13.7 [ug/mL] (The mean of the results at pH 7.4), greater than or equal to 100 mg/mL at 77 °F (NTP, 1992), Solubility in alcohol at 25 °C: about 2, in boiling alcohol: about 10%; in chloroform: about 0.1%. Practically insoluble in ether, benzene., Sol in methyl ethyl ketone, acetone, In water, 1.4X10+5 mg/L at 25 °C, 3.49e+01 g/L, Solubility in water, g/100ml at 20 °C: 12.5 |
Source
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| Record name | SID855769 | |
| Source | Burnham Center for Chemical Genomics | |
| URL | https://pubchem.ncbi.nlm.nih.gov/bioassay/1996#section=Data-Table | |
| Description | Aqueous solubility in buffer at pH 7.4 | |
| Record name | ISONIAZID | |
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| Description | CAMEO Chemicals is a chemical database designed for people who are involved in hazardous material incident response and planning. CAMEO Chemicals contains a library with thousands of datasheets containing response-related information and recommendations for hazardous materials that are commonly transported, used, or stored in the United States. CAMEO Chemicals was developed by the National Oceanic and Atmospheric Administration's Office of Response and Restoration in partnership with the Environmental Protection Agency's Office of Emergency Management. | |
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| Record name | Isoniazid | |
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| Record name | Isoniazid | |
| Source | Human Metabolome Database (HMDB) | |
| URL | http://www.hmdb.ca/metabolites/HMDB0015086 | |
| Description | The Human Metabolome Database (HMDB) is a freely available electronic database containing detailed information about small molecule metabolites found in the human body. | |
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| Record name | ISONIAZID (OBSOLETE) | |
| Source | ILO-WHO International Chemical Safety Cards (ICSCs) | |
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Vapor Pressure |
Negligible (NTP, 1992), 4.6X10-5 mm Hg at 25 °C /Estimated/ |
Source
|
| Record name | ISONIAZID | |
| Source | CAMEO Chemicals | |
| URL | https://cameochemicals.noaa.gov/chemical/20540 | |
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Mechanism of Action |
Isoniazid is a prodrug and must be activated by bacterial catalase. Specficially, activation is associated with reduction of the mycobacterial ferric KatG catalase-peroxidase by hydrazine and reaction with oxygen to form an oxyferrous enzyme complex. Once activated, isoniazid inhibits the synthesis of mycoloic acids, an essential component of the bacterial cell wall. At therapeutic levels isoniazid is bacteriocidal against actively growing intracellular and extracellular Mycobacterium tuberculosis organisms. Specifically isoniazid inhibits InhA, the enoyl reductase from Mycobacterium tuberculosis, by forming a covalent adduct with the NAD cofactor. It is the INH-NAD adduct that acts as a slow, tight-binding competitive inhibitor of InhA., Although the mechanism of action of isoniazid is unknown, several hypotheses have been proposed. These include effects on lipids, nucleic acid biosynthesis, and glycolysis. ... /It has been suggested that/ a primary action of isoniazid /is/ to inhibit the biosynthesis of mycolic acids, important constituents of the mycobacterial cell wall. Because mycolic acids are unique to mycobacteria, this action would explain the high degree of selectivity of the antimicrobial activity of isoniazid. Exposure to isoniazid leads to a loss of acid fastness and a decrease in the quantity of methanol-extractable lipid of the microorganisms., Isoniazid is bacteriostatic for "resting" bacilli but is bactericidal for rapidly dividing microorganisms. The minimal tuberculostatic concentration is 0.025 to 0.05 ug/ml. |
Source
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| Record name | Isoniazid | |
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Color/Form |
COLORLESS OR WHITE CRYSTALS, OR A WHITE, CRYSTALLINE POWDER, Crystals from alcohol | |
CAS No. |
54-85-3 |
Source
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| Record name | ISONIAZID | |
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| Record name | 4-Pyridinecarboxylic acid, hydrazide | |
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| Explanation | Unless otherwise noted, the contents of the FDA website (www.fda.gov), both text and graphics, are not copyrighted. They are in the public domain and may be republished, reprinted and otherwise used freely by anyone without the need to obtain permission from FDA. Credit to the U.S. Food and Drug Administration as the source is appreciated but not required. | |
| Record name | ISONIAZID | |
| Source | Hazardous Substances Data Bank (HSDB) | |
| URL | https://pubchem.ncbi.nlm.nih.gov/source/hsdb/1647 | |
| Description | The Hazardous Substances Data Bank (HSDB) is a toxicology database that focuses on the toxicology of potentially hazardous chemicals. It provides information on human exposure, industrial hygiene, emergency handling procedures, environmental fate, regulatory requirements, nanomaterials, and related areas. The information in HSDB has been assessed by a Scientific Review Panel. | |
| Record name | Isoniazid | |
| Source | Human Metabolome Database (HMDB) | |
| URL | http://www.hmdb.ca/metabolites/HMDB0015086 | |
| Description | The Human Metabolome Database (HMDB) is a freely available electronic database containing detailed information about small molecule metabolites found in the human body. | |
| Explanation | HMDB is offered to the public as a freely available resource. Use and re-distribution of the data, in whole or in part, for commercial purposes requires explicit permission of the authors and explicit acknowledgment of the source material (HMDB) and the original publication (see the HMDB citing page). We ask that users who download significant portions of the database cite the HMDB paper in any resulting publications. | |
| Record name | ISONIAZID (OBSOLETE) | |
| Source | ILO-WHO International Chemical Safety Cards (ICSCs) | |
| URL | https://www.ilo.org/dyn/icsc/showcard.display?p_version=2&p_card_id=1258 | |
| Description | The International Chemical Safety Cards (ICSCs) are data sheets intended to provide essential safety and health information on chemicals in a clear and concise way. The primary aim of the Cards is to promote the safe use of chemicals in the workplace. | |
| Explanation | Creative Commons CC BY 4.0 | |
Melting Point |
340.5 °F (NTP, 1992), 171.4 °C, 170-173 °C |
Source
|
| Record name | ISONIAZID | |
| Source | CAMEO Chemicals | |
| URL | https://cameochemicals.noaa.gov/chemical/20540 | |
| Description | CAMEO Chemicals is a chemical database designed for people who are involved in hazardous material incident response and planning. CAMEO Chemicals contains a library with thousands of datasheets containing response-related information and recommendations for hazardous materials that are commonly transported, used, or stored in the United States. CAMEO Chemicals was developed by the National Oceanic and Atmospheric Administration's Office of Response and Restoration in partnership with the Environmental Protection Agency's Office of Emergency Management. | |
| Explanation | CAMEO Chemicals and all other CAMEO products are available at no charge to those organizations and individuals (recipients) responsible for the safe handling of chemicals. However, some of the chemical data itself is subject to the copyright restrictions of the companies or organizations that provided the data. | |
| Record name | Isoniazid | |
| Source | DrugBank | |
| URL | https://www.drugbank.ca/drugs/DB00951 | |
| Description | The DrugBank database is a unique bioinformatics and cheminformatics resource that combines detailed drug (i.e. chemical, pharmacological and pharmaceutical) data with comprehensive drug target (i.e. sequence, structure, and pathway) information. | |
| Explanation | Creative Common's Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/legalcode) | |
| Record name | ISONIAZID | |
| Source | Hazardous Substances Data Bank (HSDB) | |
| URL | https://pubchem.ncbi.nlm.nih.gov/source/hsdb/1647 | |
| Description | The Hazardous Substances Data Bank (HSDB) is a toxicology database that focuses on the toxicology of potentially hazardous chemicals. It provides information on human exposure, industrial hygiene, emergency handling procedures, environmental fate, regulatory requirements, nanomaterials, and related areas. The information in HSDB has been assessed by a Scientific Review Panel. | |
| Record name | Isoniazid | |
| Source | Human Metabolome Database (HMDB) | |
| URL | http://www.hmdb.ca/metabolites/HMDB0015086 | |
| Description | The Human Metabolome Database (HMDB) is a freely available electronic database containing detailed information about small molecule metabolites found in the human body. | |
| Explanation | HMDB is offered to the public as a freely available resource. Use and re-distribution of the data, in whole or in part, for commercial purposes requires explicit permission of the authors and explicit acknowledgment of the source material (HMDB) and the original publication (see the HMDB citing page). We ask that users who download significant portions of the database cite the HMDB paper in any resulting publications. | |
| Record name | ISONIAZID (OBSOLETE) | |
| Source | ILO-WHO International Chemical Safety Cards (ICSCs) | |
| URL | https://www.ilo.org/dyn/icsc/showcard.display?p_version=2&p_card_id=1258 | |
| Description | The International Chemical Safety Cards (ICSCs) are data sheets intended to provide essential safety and health information on chemicals in a clear and concise way. The primary aim of the Cards is to promote the safe use of chemicals in the workplace. | |
| Explanation | Creative Commons CC BY 4.0 | |
Synthesis routes and methods I
Procedure details
Synthesis routes and methods II
Procedure details
Retrosynthesis Analysis
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| Precursor scoring | Relevance Heuristic |
|---|---|
| Min. plausibility | 0.01 |
| Model | Template_relevance |
| Template Set | Pistachio/Bkms_metabolic/Pistachio_ringbreaker/Reaxys/Reaxys_biocatalysis |
| Top-N result to add to graph | 6 |
Feasible Synthetic Routes
Q1: How does isoniazid exert its anti-tubercular effect?
A1: Isoniazid acts as a prodrug, requiring activation by a bacterial catalase-peroxidase enzyme called KatG found in Mycobacterium tuberculosis [, ]. This activation leads to the formation of an isonicotinic acyl-NADH complex, which then inhibits InhA, an enoyl-acyl carrier protein reductase essential for mycolic acid biosynthesis [, ]. Mycolic acids are crucial components of the mycobacterial cell wall, and their disruption leads to bacterial death.
Q2: Does the activation process of isoniazid play a role in drug resistance?
A2: Yes, mutations in the katG gene, particularly in codon 315, are frequently observed in isoniazid-resistant M. tuberculosis strains [, ]. These mutations can alter the enzyme's structure, affecting its ability to activate isoniazid and leading to resistance.
Q3: What is the role of the inhA gene in isoniazid resistance?
A3: Mutations in the promoter region of the inhA gene, specifically at position -15, can lead to overexpression of the InhA enzyme, reducing isoniazid's effectiveness []. This overexpression counteracts the drug's inhibitory action on mycolic acid synthesis.
Q4: How does isoniazid treatment impact collagen in the body?
A4: Isoniazid can inhibit lysyl oxidase, an enzyme essential for collagen cross-linking []. This inhibition is linked to pyridoxal phosphate depletion in the liver as pyridoxal phosphate acts as a cofactor for lysyl oxidase. The reduction in lysyl oxidase activity leads to increased collagen solubility, potentially impacting connective tissue integrity.
Q5: What is the impact of acetylator status on isoniazid treatment?
A5: Isoniazid is primarily metabolized in the liver through acetylation [, ]. Individuals are categorized as slow, heterozygous rapid, or homozygous rapid acetylators based on their genetic predisposition []. The acetylator phenotype significantly influences the rate of isoniazid elimination, impacting the drug's half-life and ultimately influencing treatment efficacy, particularly in once-weekly regimens [, ].
Q6: Does food intake affect the pharmacokinetics of isoniazid?
A6: Studies suggest that food intake, particularly a light breakfast, can influence the pharmacokinetic parameters of isoniazid, including its peak plasma concentration (Cmax) and the area under the curve (AUC) [, ]. These findings highlight the importance of considering meal timing when administering isoniazid to optimize drug exposure.
Q7: Can isoniazid be transferred through breast milk?
A7: Yes, isoniazid can transfer from circulation to breast milk in lactating women undergoing tuberculosis treatment []. Studies have determined the milk-to-plasma ratio for isoniazid, providing insights into the potential exposure of the drug to nursing infants.
Q8: How is isoniazid distributed in the body?
A8: Isoniazid exhibits good penetration into various body fluids, including cerebrospinal fluid (CSF) []. Studies have investigated the pharmacokinetic parameters associated with isoniazid transfer into CSF, particularly in patients with tuberculous meningitis. Understanding drug distribution patterns is crucial for optimizing treatment strategies, especially for infections affecting different body compartments.
Q9: What are the common adverse events associated with isoniazid treatment?
A9: The most frequently reported adverse events associated with isoniazid treatment are hepatotoxicity, gastric intolerance, and neuropathy [, , ]. The incidence of these adverse events can vary significantly. Close monitoring of liver function and neurological symptoms is crucial during isoniazid therapy.
Q10: Are there specific patient groups at higher risk of isoniazid-induced hepatotoxicity?
A10: Research suggests that certain factors might increase the risk of isoniazid-induced hepatotoxicity. These include elderly patients, individuals with pre-existing liver disease like hepatitis C infection, and those with low serum albumin levels [, ]. Careful patient selection and monitoring are essential to minimize the risk of hepatotoxicity.
Q11: Is there a treatment for isoniazid overdose?
A11: Yes, high-dose pyridoxine hydrochloride (vitamin B6) is an effective treatment for acute isoniazid overdose []. Pyridoxine, administered intravenously, can counteract the toxic effects of isoniazid and prevent potentially fatal complications.
Q12: How effective is isoniazid prophylaxis in individuals exposed to isoniazid-resistant tuberculosis?
A12: There have been reports of isoniazid chemoprophylaxis failure in preventing active pulmonary tuberculosis and tuberculin conversion in individuals exposed to isoniazid-resistant strains of M. tuberculosis []. These observations highlight the limitations of isoniazid prophylaxis in such situations and emphasize the need to consider alternative prophylactic agents.
Q13: What analytical methods are commonly used to determine isoniazid concentrations?
A13: Several analytical techniques have been employed to quantify isoniazid concentrations in various matrices. These include spectrophotometry, high-performance liquid chromatography (HPLC), and chemiluminescence-based methods [, ]. Each method offers advantages and limitations in terms of sensitivity, selectivity, and practicality.
Q14: Are there specific formulation strategies to enhance isoniazid delivery or stability?
A14: Yes, researchers have explored different formulation approaches to improve isoniazid delivery and stability. These include the development of slow-release preparations to sustain drug release, enhance patient compliance, and potentially reduce the dosing frequency [, ].
Q15: What is the historical significance of isoniazid in tuberculosis treatment?
A15: The discovery and introduction of isoniazid marked a significant milestone in the fight against tuberculosis. This potent drug, particularly when combined with other anti-tuberculosis agents, revolutionized treatment strategies and significantly improved patient outcomes. Isoniazid remains a cornerstone of tuberculosis treatment regimens worldwide, underscoring its enduring importance in global health.
Q16: What are the potential areas for future research on isoniazid?
A16: Future research endeavors could focus on several aspects, including:
- Developing novel isoniazid derivatives with improved efficacy and safety profiles [].
- Exploring alternative drug delivery systems to enhance targeted delivery and minimize side effects [].
- Investigating new strategies to combat emerging drug resistance and ensure the long-term effectiveness of isoniazid-containing regimens [, ].
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Tenga en cuenta que todos los artículos e información de productos presentados en BenchChem están destinados únicamente con fines informativos. Los productos disponibles para la compra en BenchChem están diseñados específicamente para estudios in vitro, que se realizan fuera de organismos vivos. Los estudios in vitro, derivados del término latino "in vidrio", involucran experimentos realizados en entornos de laboratorio controlados utilizando células o tejidos. Es importante tener en cuenta que estos productos no se clasifican como medicamentos y no han recibido la aprobación de la FDA para la prevención, tratamiento o cura de ninguna condición médica, dolencia o enfermedad. Debemos enfatizar que cualquier forma de introducción corporal de estos productos en humanos o animales está estrictamente prohibida por ley. Es esencial adherirse a estas pautas para garantizar el cumplimiento de los estándares legales y éticos en la investigación y experimentación.
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